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Figure 1 | Virology Journal

Figure 1

From: Uncoupling GP1 and GP2 expression in the Lassa virus glycoprotein complex: implications for GP1 ectodomain shedding

Figure 1

Graphic representation of LASV GPC, GP1, and GP2 constructs employed in the expression of glycoproteins in mammalian cells. The LASV Josiah GPC gene was the backbone for all glycoprotein expression constructs. (A. i) The 58 amino-acid signal peptide (SP) which is post-translationally cleaved by SPase precedes the 201 amino acid GP1 ORF (59 – 259). The GP2 ORF spans amino acids 260 – 491. The GP2 gene contains an ectodomain of 168 amino acids, followed by a 24 amino acid transmembrane (TM) domain, and a 40 amino acid intracellular (IC) domain. The cleavage positions of SPase and SKI-1/S1P proteases are indicated by arrows. The relative position of 7 N-linked glycosylation sites in GP1 and 4 in GP2 are indicated by Y symbols. Constructs for expression of GPC-FLAG (A. ii), GP1 (B), GP2 and soluble GPC (C), are noted. The pcDNA3.1(+) plasmid background was used for expression of glycoprotein constructs (D).

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Virology Journal

ISSN: 1743-422X