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Table 1 Universal primers Flav100F and Flav200R developed and tested in this study.

From: Universal primers that amplify RNA from all three flavivirus subgroups

Primer

Sequence

Binding on YF ref (NC_002031)

  

Start

End

Flav100F

AAY TCI ACI CAI GAR ATG TAY

8276

8296

Flav200R

CCI ARC CAC ATR WAC CA

9062

9078

  1. N = A+C+G+T, R = A+G, W = A+T, Y = C+T.
  2. These primers work with Inosines or N included during primer synthesis.