Figure 1

Alignment of the deduced ARV138 and ARV176 λB amino acid sequences. All 14 currently available homologous ARV λB, MRV λ3, and AqRV VP2 proteins (determined for each clone shown in Table 1) were aligned, both by T-Coffee [50] (data not shown) and by Clustal-W [49], with only minor differences in the alignments created by different gap penalties (data not shown). Only the two most-distant ARV, MRV, and AqRV sequences (see text for details) are shown for clarity. Clones are: MRV – T1L (GenBank No. NC_004271) and T2J (GenBank No. NC_004272); ARV – ARV138 (GenBank No. EU707935) and ARV176 (GenBank No. EU707936); AqRV – Grass Carp reovirus (GCRV) (GenBank No. AF260512) and Chum Salmon reovirus (CSRV) (GenBank No. NC_007583). Amino acid residues that are identical in at least four of the sequences are indicated by black background shading. The single letter amino acid code is used. Previously identified polymerase domains (labeled I – III) [56] are indicated with solid horizontal lines above the sequences. Amino acid residues that are completely conserved in all 14 sequences are indicated by closed circles, and the GDD motif found in all polymerases is indicated by open circles, shown above the sequences.