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Figure 4 | Virology Journal

Figure 4

From: Identification and characterization of a new E3 ubiquitin ligase in white spot syndrome virus involved in virus latency

Figure 4

WSSV403 can interact with a shrimp protein phosphatase. WSSV403 was found to interact with shrimp PPs in yeast two hybrid. Cotransformed yeast was screened on -Leu-Trp SD plates (A) and -Leu-Trp-His-Ade plates with x-α-gal (B). 1, yeast cotransformed with pGBK-403 and pGAD-PPs; 2, yeast cotransformed with pGBK-403 and pGAD; 3, yeast cotransformed with pGBK and pGAD-PPs; 4, yeast cotransformed with positive plasmids from the kit (Clontech). (C) Pull-down assays with WSSV403 and GST-PPs. Soluble protein complexes were bound to GST beads and washed under high stringency condition before SDS-PAGE and immunoblot detection of His6-WSSV403. Immunoblots were performed with anti-His6 or anti-GST.

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Virology Journal

ISSN: 1743-422X