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Table 1 Table 1

From: Functional characterization of the vaccinia virus I5 protein

Virus

Primer Name

Primer Sequence *

I5V5

  
 

PN 5'

5' ATGGATCC GGAAGGGTATCTATACTTATAG 3'

 

I5V5 3'

5' TCCCAACAAAGGGTTAGGGATAGGTTTACCACT 3'

 

I5V5 5'

5' CCTAACCCTTTGTTGGGACTCGACAGTACTTAA 3'

 

PN 3'

5' ATGGATCC GTTGAATAAATCCTCCATC 3'

 

SCRN 5'

5' GTACGCTACGTACGTCAAATCCC 3'

 

SCRN 3'

5' GGCATAATCCGGATGTTGTGTAG 3'

VΔind I5V5

  
 

1

5' GGGGATCC AAGCTT CTAGGACTTTGTCAC 3'

 

B

5' CTCTATCACTGATAGGGATATTTATATCTAAAAATTAGATC 3'

 

C

5'CCCTATCAGTGATAGAGAATGGTGGATGCTATAAC 3'

 

3'

5'ATGGATCC atcgatTTAAGTACTGTCGAGTCCCAACAAAGG GTTAGGGATAGGTTTACCACTTTTCATTAATAGGG 3'

vΔI5

  
 

I4 pUCneo Asp

5' GCGCCCggtacc GAATAAATCCTCCATC 3'

 

I4 pUCneo Bam

5' CGGGATCC GTACGTAAAATCCCTATT 3'

 

I6pUCneo Bam/Hind

5' CGGGATCC AAGCTT CTAAAAATTAGATCAAAG 3'

 

I6 pUCneo Xba

5' ATTCTAGA GGCGGTGTGGATTTC 3'

  1. *The restriction enzyme sites encoded in the primers are noted as follows: BamHI, bold; HindIII, italics; ClaI, lower case; Asp718, lowercase italics; XbaI, small caps.
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Virology Journal

ISSN: 1743-422X