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Figure 2 | Virology Journal

Figure 2

From: Effects of vaccinia virus uracil DNA glycosylase catalytic site and deoxyuridine triphosphatase deletion mutations individually and together on replication in active and quiescent cells and pathogenesis in mice

Figure 2

Virus replication and DNA synthesis in BS-C-1 cells. Confluent 6 well plates of BS-C-1 cells were infected with WR and recombinant viruses at a multiplicity of 5. The infected cells were harvested at the indicated hours post infection (hpi) to determine virus titers and viral DNA. (A) Virus titers were determined on BS-C-1 cells and plotted as PFU/ml. All experiments were carried out in triplicate. Average titers are shown; bars representing standard error of the mean could not be printed because of their very close spacing. (B) Viral DNA was determined by blot hybridization and quantified with a phosphorImager. Experiment represents average of duplicate experiments.

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Virology Journal

ISSN: 1743-422X