Figure 3

NiV infection of EB2 expressing PAE cells (PAEC-EB2). (A) PAEC and PAEC-EB2 cells were immunostained for EB2 as described in the legend to Fig. 2B. Magnification, ×100. (B) PAEC and PAEC-EB2 were infected with NiV at a MOI of 0.2. At 24 h p.i., cells were fixed and immunostaining was performed as described in the legend to Fig. 1A. Magnification, ×20. (C) 48 h p.i., mRNA was isolated from the cell supernatant, and RT-PCR was performed with NP-specific primers.