EB2 and EB3 expression in model EC. (A) EC were cultured on coverslips and immunostaining was performed using recombinant EphB4/Fc and rhodamine-conjugated secondary antibodies. Nuclei were visualized by DAPI staining. Magnification, ×100. (B) mRNA was extracted from 5 × 105 cells by standard procedures and subjected to RT-PCR with EB2-specific primers. (C) RT-PCR with EB3-specific primers.