NiV infection and NiV glycoprotein-mediated cell-to-cell fusion in different model EC. PBMEC, HBMEC, PAEC, MyEnd, Ea.hy 926 and control Vero and HeLa cells were infected with NiV at a MOI of 0.2. (A) At 24 h p.i., cells were fixed, incubated with a NiV-specific guinea pig antiserum and visualized with rhodamine-conjugated secondary antibodies. Nuclei were counterstained with DAPI. (B) At 48 h p.i., viral RNA was isolated from supernatants of infected cells. RT-PCR was performed using NP-specific primers (NPfor binds at bp 1160–1179 and NPrev binds at bp 1271–1292). (C) HeLa cells were cotransfected with plasmids encoding the NiV glycoproteins F and G and were incubated at 33°C. 22 h after transfection, control cells and the different EC types were overlayed with NiV F- and G-expressing HeLa cells. 24 h later, cell-to-cell fusion was visualized by Giemsa staining. Magnification, ×20.