Figure 3From: Plaque assay for human coronavirus NL63 using human colon carcinoma cellsPlaque assay for hCoV-NL63 on CaCo-2 cells using different overlays. HCoV-NL63 was serially diluted on CaCo-2 cells (10e-1 until 10e-5). After 1 h of virus adsorbtion different overlays were added. After 5 days cells were fixed with 4% formaldehyde and stained with 0.2% crystal violet solution. A) carboxymethyl-cellulose; B) agarose; C) Avicel.Back to article page