Figure 2
From: Plaque assay for human coronavirus NL63 using human colon carcinoma cells
Growth kinetics of hCoV-NL63 on LLC-MK2 and CaCo-2 cells. 25 cm2 flasks of LLC-MK2 or CaCo-2 cells were infected at multiplicities of infection of 0.005 for 1 h, washed twice with PBS, and subsequently supplied with 10 mL DMEM. Samples were taken daily from day 0 to 7 (except day 4) and analyzed by real time RT-PCR. Error bars indicate ranges of three independent experiments.