Figure 5
From: The YPLGVG sequence of the Nipah virus matrix protein is required for budding
NiV M release is insensitive to VPS4A inhibition. (A) Cells were transfected with expression plasmids for SV5 N, M, F and HN, or NiV M, along with either wild-type or the dominant-negative VPS4A, followed by 35S-metabolic labeling. Released protein was pelleted through 10% sucrose, as described in the Methods, and proteins derived from cell lysates (L) and culture supernatants (S) were immunoprecipitated with either rabbit anti-SV5 polyclonal serum or MAb F45G5 (anti-NiV M) and analyzed by SDS-PAGE followed by autoradiography. (B) Expression of VPS4A was detected in cell lysates from the material analyzed in A by immunoprecipitation with rabbit polyclonal antiserum against GFP, followed by SDS-PAGE and autoradiography.