NiV sequence can rescue Ebola VP40-induced cellular morphology. (A) The L-domain and flanking sequence of VP40 (underlined) was replaced with a sequence derived from NiV M (shaded) containing the YPLGVG sequence. (B) COS-1 cells were transfected with plasmids encoding VP40 wt, VP40 ΔPT/PY, or VP40-NiV. Cells were fixed 20–24 h post-transfection, permeablilized, and incubated with mouse anti-VP40 MAb followed by Alexa Fluor 488 donkey anti-mouse antibody and analyzed by confocal microscopy. The VP40-NiV immunofluorescence experiment was performed separately; all images are representative.