Table 1 Primer sequences used to construct pGL3-hsc70B plasmids
From: Ex vivo promoter analysis of antiviral heat shock cognate 70B gene in Anopheles gambiae
Primers | Primer sequence (5' to 3') | Position | Usage |
|---|---|---|---|
AngaHsc_F1 | CCCGAGCTC GATGGTCACAAATGTTTCACAGG | -2599 | Forward primer to construct pGL3-2.6k |
AngaHsc_F2 | CCCGAGCTC CTTTCTAGAAAAGTGTGGAAAGAACAG | -2150 | Forward primer to construct pGL3-2.2k |
AngaHsc_F3 | CCCGAGCTC GGGTAATGGTCCAATGGGTC | -1624 | Forward primer to construct pGL3-1.6k |
AngaHsc_F4 | CCCGAGCTC TGTGAAATGTCCTAATTTTTTGCC | -950 | Forward primer to construct pGL3-0.9k |
AngaHsc_F5 | CCCGAGCTC GCATCATGCGTTAGGTCTCAG | -332 | Forward primer to construct pGL3-0.3k |
AngaHsc_R | CCGCTCGAG CTGCGAACACGCAACACAC | -1 | Reverse primer to construct all plasmids |