Table 1 Primers for detection of TYLCV in infected plants, sequencing of TYLCV clones, and relative quantification of TYLCV with quantitative PCR (Q-PCR)
From: A novel cloning strategy for isolating, genotyping and phenotyping genetic variants of geminiviruses
Primer code | Primer sequence | Final concentration used |
|---|---|---|
Detection of TYLCV with PCR (600 bp amplicon) | ||
Ty 2353+ | CTGAATGTTTGCATGGAAATGTGC | 200 nM |
Ty321- | GGTCGCTTCGACATARTCACG | 200 nM |
Sequencing of TYLCV cloned sequences | ||
pGreen1589 (+) | CACGACGTTGTAAAACGACG | |
pG1825(-) | CACAGGAAACAGCTATGACC | |
579 (+) | GATGTTACTCGTGGATCTGG | |
1141 (+) | CATGATCAACTGCTCTGATTAC | |
1741(+) | GGGCTTCCCGTACTTTGTG | |
2321(+) | TGGATTTAGCTCCCTGAATG | |
TYLCV primers for Q-PCR (176 bp amplicon) | ||
Ty 2164+ | CTAAGAGCCTCTGACTTACTGC | 200 nM |
Ty 2339- | AACATTCAGGGAGCTAAATCCAG | 200 nM |
Actin2 gene primers for Q-PCR (148 bp amplicon) | ||
Act 1+ | CCCRGAGGTHCTCTTCCARC | 200 nM |
Act 148- | TMCGRTCAGCAATACCAGGG | 200 nM |