Figure 4From: A novel cloning strategy for isolating, genotyping and phenotyping genetic variants of geminivirusesConstruction of pGreen-SL- Not I. (A) Site-directed mutagenesis at the end of the stem loop to generate a NotI site in the TYLCV-Mld clone. The underlined bold A nucleotide in the loop indicates the origin of replication. (B) Oligonucleotide sequence used to insert the stem loop shown in (A) in the multiple cloning site of the vector pGreen.Back to article page