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Figure 1 | Virology Journal

Figure 1

From: A novel cloning strategy for isolating, genotyping and phenotyping genetic variants of geminiviruses

Figure 1

Schematic representation of the infectious construct p1.01TYLC and position of mutations detected in the progeny. The schema represents TYLCV-Mild-NotI cloned in the binary plasmid vector pGreen, The hatched boxes represent the vector; white boxes represent the intergenic region of TYLCV-Mld-NotI, and the white horizontal arrows represent viral genes as indicated. The stem loop, including the NotI cloning site, is represented by a vertical bar topped by a white circle. Vertical arrows and associated numbers indicate the position of the mutations detected in the progeny full-length clones. Changes in nucleotide and amino acid sequences detected at the positions indicated are shown below the scheme (original/mutated).

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