Figure 1
From: A novel cloning strategy for isolating, genotyping and phenotyping genetic variants of geminiviruses
Schematic representation of the infectious construct p1.01TYLC and position of mutations detected in the progeny. The schema represents TYLCV-Mild-NotI cloned in the binary plasmid vector pGreen, The hatched boxes represent the vector; white boxes represent the intergenic region of TYLCV-Mld-NotI, and the white horizontal arrows represent viral genes as indicated. The stem loop, including the NotI cloning site, is represented by a vertical bar topped by a white circle. Vertical arrows and associated numbers indicate the position of the mutations detected in the progeny full-length clones. Changes in nucleotide and amino acid sequences detected at the positions indicated are shown below the scheme (original/mutated).