Schematic representation of the infectious construct p1.01TYLC and position of mutations detected in the progeny. The schema represents TYLCV-Mild-NotI cloned in the binary plasmid vector pGreen, The hatched boxes represent the vector; white boxes represent the intergenic region of TYLCV-Mld-NotI, and the white horizontal arrows represent viral genes as indicated. The stem loop, including the NotI cloning site, is represented by a vertical bar topped by a white circle. Vertical arrows and associated numbers indicate the position of the mutations detected in the progeny full-length clones. Changes in nucleotide and amino acid sequences detected at the positions indicated are shown below the scheme (original/mutated).