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Figure 1 | Virology Journal

Figure 1

From: Non coding extremities of the seven influenza virus type C vRNA segments: effect on transcription and replication by the type C and type A polymerase complexes

Figure 1

Transcription/replication of (-) sense model RNA templates derived from the type C genomic segments. 293T cells were transfected in duplicate with the four pHMG plasmids encoding the polymerase complex of influenza virus type C (closed bars) or type A (open bars), together with 100 ng of plasmids expressing CAT reporter vRNA-like templates derived from the influenza virus type C genomic segments as indicated. At 24 h post-transfection, cell extracts were prepared and the levels of CAT were determined as described in Methods. The results are expressed as the mean +/- SD of duplicate samples from one experiment representative of two independent experiments for A, C and D and as the mean +/- SD from two experiments for B. The names of the pC/PRCAT/plasmids were shortened to the name of the virus segment or of the mutant. (A) vRNA-like templates with wild-type NC regions. (B-C-D) respectively, NS-, PB2-, M- like vRNA templates with mutations in the 5'NC sequence.

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Virology Journal

ISSN: 1743-422X