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Figure 3 | Virology Journal

Figure 3

From: Neutralizing human monoclonal antibody against H5N1 influenza HA selected from a Fab-phage display library

Figure 3

H5N1-VLP neutralization assays with Fab derived human IgGs. H5N1-VLP were produced in HEK293 cells and the media incubated with 5 ug/ml of the indicated IgG for 60 min prior to addition to MDCK cells. (A) Initial validation of the VLP neutralization assay was performed using IN597- and INDO05-VLP with and without VN04-2, fusion of the VLP with MDCK was visualized by immunofluorescence microscopy. (B) Testing of the isolated IgG was performed against IN597-VLP and fusion of the VLP with MDCK was determined by measuring the number of cells producing GFP by flow cytometry with the control assay with no IgG taken as 100%. The humanized protective antibody VN04-2 was used as a positive control.

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