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Figure 1 | Virology Journal

Figure 1

From: Neutralizing human monoclonal antibody against H5N1 influenza HA selected from a Fab-phage display library

Figure 1

Analysis of HA-FO and H5-VLP. (A) The indicated HA foldons from recombinant baculovirus were collected and allowed to incubate with Talon resin. Following elution, equal amounts were treated with trypsin to check for correct folding, heat denaturation was used as a control for trypsin activity. Proteins were resolved on 12% SDS-PAGE and stained with coomassie. Mw markers (sizes in kDa, left) and the bands representing HA0, HA1 and HA2 are indicated. (B) H5-VLP were released from HEK293 cells by addition of exogenous NA, the VLP pellet after sucrose cushion purification was resolved on 12% SDS-PAGE. Immunoblots were probed with α-HA (VN04-2).

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