Agarose gel electrophoresis showing clone p GNPV-95 after linearization with Nde I, and PCR confirmation. Lanes 1 and 2 show p GEM-T and p GNPV-95 after digestion with Nde I, respectively. Lanes 3 and 4 show Lambda DNA/Hind III Marker. Lanes 5, 6 and 7 show the 405 bp amplified DNA segment from Spli NPV (as positive control), from E. coli harbouring p GNPV-95 and PCR mix without DNA (as negative control), respectively. The size of the bands is shown in bp.