Figure 4
From: Adaptation to cell culture induces functional differences in measles virus proteins
Effect of mutations in the H and M proteins of MeV on quantitative fusion. (A) fusion produced by H and F proteins, (B) inhibition of fusion by co-expressed M protein. Vero/hSLAM cells were infected with MVAT7 and transfected with the indicated plasmids. The amount of transfected DNA was kept constant through the addition of pTM1 vector. β-galactosidase protein production in cytoplasmic extracts of quadruplicate samples was measured as described in the Methods section. The amount of β-galactosidase protein measured in the wells transfected with pTM1-D87-wt F and pTM1-D87-wt H was set to 100% in each panel. Each panel shows the average of three independent experiments. Error bars denote one standard deviation. In panels (A) and (B) F indicates D87-wt F, in panel (B), H indicates D87-wt H. (**: P ≤ 0.001)