Interaction between UL7 and ANT2 in mammalian cells. (A) Photograph of an immunoblot of electrophoretically separated lysates of COS-7 cells transfected with pCMV(f)UL7 (lane 1) or pCMV(f)ANT (lane 2) and subjected to immunoblotting with the antibody to the flag epitope. (B) COS-7 cells transfected with the indicated expression plasmids were immunoprecipitated with antibody to the UL7. The immunoprecipitates were subjected to electrophoresis on a denaturing gel, transferred to a PVDF sheet, and reacted with the flag antibody. Four percent of the COS-7 whole cell extracts (WCE) input to the immunoprecipitation reactions for lanes 1 and 2 were loaded into lanes 3 and 4, respectively.