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Figure 3 | Virology Journal

Figure 3

From: The product of the Herpes simplex virus 1 UL7 gene interacts with a mitochondrial protein, adenine nucleotide translocator 2

Figure 3

Identified host proteins interact with UL7 by using MEF purification method. (A) Schematic diagrams of the expression plasmid containing UL7 tagged with the myc, TEV protease, and flag. (B) Photograph of an immunoblot of electrophoretically separated lysates of COS-7 cells transfected with pcDNA MEF (lane 1) or pMEF7 (lane 2) and subjected to immunoblotting with the mouse monoclonal antibody to the flag epitope. (C) Photograph of electrophoretically separated lysates of 293T cells. 293T cells transfected with pMEF (lane 1) or pcDNA MEF (lane 2) were harvested, lysed, and immunoprecipitated as described in Materials and Methods. The proteins bound to UL7 were purified and separated with 10% SDS-page and silver-stained (lane 1). The bands surrounded by the white rectangle and indicated by the arrowhead were subjected to a mass spectrometry experiment. (D) Peptide sequence of ANT2. The sequences detected by mass spectrometry and specific for ANT2 are shown in bold type. The sequences conserved in ANT 1~3 or ANT1~4 are shown in bold type and underlined.

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