Virus titer in blood and brain from mice at different hours post infection (HPI). The serum and brain supernatant from infected as well as control mice was titrated in Vero E6 cells. The end point was determined by the reciprocal of highest dilution which showing CPE. The tissue culture infective dose 50 (TCID50) per ml (TCID50/ml) was calculated by Reed and Munch formula. The values are Mean ± SE of two experiments. Each experiment tissues were collected from three different mice from each group and processed separately.