Genus | Species | Infected region | HPV type | pI-value | Charge at pH 7.4 |
|---|
Alpha-papillomavirus | 1 | Mucosa | 32 | 8.38 | 5.2 |
| | 2 | Cutaneous (Mucosa) | 10 | 7.65 | 1.02 |
| | 3 | Mucosa | 61 | 8.2 | 4.88 |
| | 4 | Cutaneous (Mucosa) | 2 | 6.87 | -1.8 |
| | 5 | Mucosa | 26 | 8.38 | 5.24 |
| | 6 | Mucosa | 53 | 8.59 | 6.3 |
| | 7 | Mucosa | 18 | 8.35 | 7.85 |
| | 8 | Mucosa (Cutaneous) | 7 | 7.46 | 0.2 |
| | 9 | Mucosa | 16 | 7.95 | 2.98 |
| | 10 | Mucosa | 6 | 8.55 | 7.08 |
| | 11 | Mucosa | 34 | 8.3 | 6.68 |
| | 13 | Mucosa | 54 | 8.5 | 6.15 |
Beta-papillomavirus | 1 | Cutaneous | 5 | 6.59 | -2.74 |
| | 2 | Cutaneous | 9 | 5.88 | -6.76 |
| | 3 | Cutaneous | 49 | 6.01 | -4.94 |
Gamma-papillomavirus | 1 | Cutaneous | 4 | 6.34 | -4.43 |
| | 2 | Cutaneous | 48 | 5.57 | -7.53 |
| | 3 | Cutaneous | 50 | 6.02 | -5.57 |
| | 4 | Cutaneous | 60 | 7.14 | -0.71 |
Mu-papillomavirus | 1 | Cutaneous | 1 | 6.63 | -1.54 |
| | 2 | Cutaneous | 63 | 6.56 | -2.43 |
Nu-papillomavirus | 1 | Cutaneous | 41 | 6.13 | -6.71 |
- The HPV types displayed are the HPV type species according to[2]. The other HPVs in the species had similar pI as the type species (data not shown). The theoretical isoelectric point (pI) calculations in this study were based solely on protein sequence and did not take 3D-aspects or residue-residue interactions into consideration. To attain a prediction of the pI of the protein the theoretical charge was calculated at every pH between 1 and 13, with an increment of 0.01. The pH with the total charge closest to zero was used as the pI of the protein. The theoretical charge of a sequence at any given pH was determined by the frequency of a few amino acids. Lysine, arginine, histidine and the N-terminal residue contributed positively to the over all charge of a sequence, whereas aspartic acid, glutamic acid, cysteine, tyrosine as well as the C-terminal residue contributed negatively. All other types of amino acids were for this purpose considered neutral. To attain the overall charge of the protein, the total contributing charge for each of the positive amino acids was summarized and the total contributing charge for all negative amino acids was subtracted http://www.acc.umu.se/~wibom/virology/cgi-bin/pI_predictor.cgi. The pKa-constants we employed for our calculations were kindly shared to us by the European Molecular Biology Laboratory.
