RSV stimulation of SOCS1, SOCS3, IFNα and IFNβ mRNA expression. MLE-15 cells were mock-infected or infected with WT, ΔG, or ΔNS1/2 virus at a multiplicity of infection (MOI) of 1 for 24 h (A) or 48 h (B). Cells were harvested at the times indicated. SOCS1, SOCS3, IFNα and IFNβ mRNA expression were measured by real-time PCR. Transcript levels were normalized to hypoxanthine guanine phosphoribosyl transferase (HPRT) expression and calibrated to the mock condition. Data is presented as fold-differences in gene expression relative to mock-infected MLE-15 cells. Differences in gene fold expression between virus infection groups were evaluated by Mann-Whitney U test and noted as significant as denoted by an asterisk. Data are shown as means ± standard errors (SE) of the means.