Figure 10

Engagement of Integrin Receptors with a Non-Infectious Peptide Suppresses CYP3A2 in Primary Rat Hepatocytes. Treatment with a peptide rich in RGD sequences at a concentration known to reduce adenovirus transduction by over 80% in culture (1.5 mg/ml, [58]) reduced CYP3A2 activity in primary hepatocytes by 60%. A peptide that differed by only one amino acid residue at the same concentration (RGE, 1.5 mg/ml) that does not block/engage integrin receptors did not affect CYP activity. Cells were also treated with bacterial lipopolysaccharide (LPS, 10 μg/ml) and cyclosporine A (CsA, 5 μg/ml), compounds that also suppress CYP3A2 [59, 60], to confirm our results. Values are presented as the mean ± standard error of 3 replicates for each treatment group. Statistical significance was determined between individual treatment groups and vehicle controls by one-way analysis of variance with a Bonferroni/Dunn post-hoc test. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.