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Figure 4 | Virology Journal

Figure 4

From: Hantaviruses and TNF-alpha act synergistically to induce ERK1/2 inactivation in Vero E6 cells

Figure 4

Hantaviruses and TNF-α synergistically inhibit ERK1/2 activity. To evaluate the role of TNF-α in hantavirus-mediated ERK1/2 inactivation, Vero E6 cells infected with different hantaviruses (see Figure 3) were incubated with (+) or without (-; same samples as in Figure 3) TNF-α (20 ng/ml). Fresh TNF-α was added together with fresh cell culture medium once a week. Cell lysates (50 μg protein) were immunoblotted for detection of phosphorylated ERK1/2 (p-ERK1/2) or total ERK1/2. Bands were subjected to intensity analysis (ImageJ software; http://rsb.info.nih.gov/ij) and the amount of p-ERK1/2 related to mock sample without TNF-α treatment at 14 and 25 days post infection (p.i.).

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