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Figure 3 | Virology Journal

Figure 3

From: Evidence that the Nijmegen breakage syndrome protein, an early sensor of double-strand DNA breaks (DSB), is involved in HIV-1 post-integration repair by recruiting the ataxia telangiectasia-mutated kinase in a process similar to, but distinct from, cellular DSB repair

Figure 3

Reintroduction of normal NBS1 cDNA into NBS1-deficient cells restores HIV-1 transduction efficiency. NBS1-deficient cells were transfected with a plasmid encoding normal NBS1 cDNA or an empty vector plasmid. One day post-transfection, cells were infected with the HIV-1-based vector carrying the lac Z reporter. Cells were then stained eight days post-infection using a β-galactosidase assay and transduced (blue) cells were counted. c – control (cells transfected) with the empty vector, NBS1 – cells transfected with the plasmid carrying the normal NBS1 gene. The error bars represent standard deviation, p = 0.037.

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