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Figure 1 | Virology Journal

Figure 1

From: Evidence that the Nijmegen breakage syndrome protein, an early sensor of double-strand DNA breaks (DSB), is involved in HIV-1 post-integration repair by recruiting the ataxia telangiectasia-mutated kinase in a process similar to, but distinct from, cellular DSB repair

Figure 1

NBS1 associates with viral DNA and is required for recruitment of ATM but not ATR. (A) Chromatin immunoprecipitation of infected NBS1-deficient and control cells. To establish if NBS1, ATM, and/or ATR associate with viral DNA, normal and NBS1-deficient cells were infected with the HIV-1-based vector at an m.o.i. of 0.1 and chromatin immunoprecipitation was performed with anti-NBS1, anti-ATM and anti-ATR antibodies as described in the Experimental Procedures. m – mock, uninfected cells. The immunoprecipitating antibody is indicated on the left side of the photograph of the gel. (B) Chromatin immunoprecipitation of infected NBS1-deficient and control cells, which were transfected with the normal NBS1 gene. Control and NBS1-deficient cells were transfected with the NBS1-coding plasmid or an empty vector. 48 hrs post-transfection, cells were infected with the HIV-1-based vector at an m.o.i. of 0.1 and chromatin immunoprecipitation was performed 24 hrs later with anti-NBS1 and anti-ATM antibodies as described in the Experimental Procedures. m – uninfected cells, v – cells infected with the HIV-1-based vector, N – cells transfected with the normal NBS1 gene and infected with the HIV-1-based vector, c – cells transfected with the empty plasmid vector and infected with the HIC-1-based vector.

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Virology Journal

ISSN: 1743-422X