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Figure 1 | Virology Journal

Figure 1

From: Neuraminidase activity provides a practical read-out for a high throughput influenza antiviral screening assay

Figure 1

NA activity can be used to quantify virus. A/Wisconsin/67/2005 was serially diluted in PBS and 50 μl incubated with an equal volume of 20 μM MU-NANA for 1 hour at 37°C. Stop solution was added before reading fluorescence. The signal to background ratio at each concentration is shown. Under these conditions enzyme activity reaches a plateau with > 5 × 105 TCID50 due to limiting substrate.

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Virology Journal

ISSN: 1743-422X