Skip to main content
Figure 11 | Virology Journal

Figure 11

From: Discovery of frameshifting in Alphavirus 6K resolves a 20-year enigma

Figure 11

Immunofluorescence of SFV-infected cells showing location of 6K and TF proteins. Green fluorescence indicates Abs binding to target peptides. Cell nuclei are stained blue. Cells fixed in acetone are permeabilized, allowing intracellular Ab staining. Cells fixed in 4% PFA are not permeabilized, thus only allowing Abs to bind to peptides at the cell surface. Cells were infected with WT SFV4 virus (WT), the TF knockout mutant (TF-), or are non-infected controls (-). TF- serves as an additional control for Ab-TF-C. See also Additional file 2.

Back to article page