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Figure 1 | Virology Journal

Figure 1

From: Impairment of the CD8+ T cell response in lungs following infection with human respiratory syncytial virus is specific to the anatomical site rather than the virus, antigen, or route of infection

Figure 1

Examples of primary data of flow cytometry analysis of tetramer/pentamer+CD8+ and IFNγ+CD8+ cells from the lungs and the spleens of individual mice. Mice were mock-infected or infected as indicated below the plots on days 0 and 28. The animals were sacrificed 8 days later (day 36) and lungs and spleens were collected. PMC and splenocytes were isolated and stained with MHC class I tetramer or pentamer complexes specific for an RSV or influenza virus epitope or stimulated in vitro with the epitope-specific peptide, stained for intracellular IFNγ and analyzed by flow cytometry. Percentages relative to total CD8+ cells are shown for various cell populations. The data are from the experiment shown in Table 1.

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