Figure 1From: Impairment of the CD8+ T cell response in lungs following infection with human respiratory syncytial virus is specific to the anatomical site rather than the virus, antigen, or route of infectionExamples of primary data of flow cytometry analysis of tetramer/pentamer+CD8+ and IFNγ+CD8+ cells from the lungs and the spleens of individual mice. Mice were mock-infected or infected as indicated below the plots on days 0 and 28. The animals were sacrificed 8 days later (day 36) and lungs and spleens were collected. PMC and splenocytes were isolated and stained with MHC class I tetramer or pentamer complexes specific for an RSV or influenza virus epitope or stimulated in vitro with the epitope-specific peptide, stained for intracellular IFNγ and analyzed by flow cytometry. Percentages relative to total CD8+ cells are shown for various cell populations. The data are from the experiment shown in Table 1.Back to article page