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Figure 4 | Virology Journal

Figure 4

From: Subcellular forms and biochemical events triggered in human cells by HCV polyprotein expression from a viral vector

Figure 4

Immunogold electron microscopy analysis of the localization of HCV proteins in VT7-HCV 7.9 infected HeLa cells. HeLa cells infected with VT7-HCV7.9 in the presence or absence of IPTG were chemically fixed, quickly frozen in liquid propane and then processed at low temperature in Lowicryl K4M resin. Immunogold labelling was performed with different antibodies. A: Cells infected with VT7-HCV7.9 in the absence of IPTG reacted with a serum from an HCV-infected patient.B: Cells infected with VT7-HCV7.9 in the presence of IPTG reacted with a serum from an HCV-infected patient C: Cells infected with VT7-HCV7.9 in the presence of IPTG reacted with a rabbit polyclonal anti-NS4B. D: Cells infected with VT7-HCV7.9 in the presence of IPTG reacted with a rabbit polyclonal anti-NS5A. E: Cells infected with VT7-HCV7.9 in the presence of IPTG reacted with a mouse monoclonal antibody anti-PDI. Note: Electron dense structures in membranous webs (EDS); mitochondria (m), immature virus (IV), intracellular mature virus (IMV), nucleus (N) and Vacuole (V). Bar: 250 nm.

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