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Figure 2 | Virology Journal

Figure 2

From: Comparative genomics of Bacillus thuringiensis phage 0305φ8-36: defining patterns of descent in a novel ancient phage lineage

Figure 2

Main structure-encoding region of 0305φ8-36 showing similarities to BtI1 and paralogous domains. The figure was modified from Gbrowse output as described in the methods. Phage 0305φ8-36 orfs are color coded as in Figure 1. BtI1 orfs are color coded as follows: Green – N terminus of a BtI1 gene. Shades of red from bright to pale indicate assignment of homology with increasing reliance on positional information as described in the methods. The size of a connector dropping below the chain of matches indicates the amount of DNA missing in BtI1 versus 0305φ8-36. A triangle above the chain of matches indicates the amount of DNA in BtI1 in excess over 0305φ8-36. Boundaries of BtI1 frames marked with an asterisk were revised over those indicated in GenBank. Red angle brackets fuse two BtI1 orfs by correcting a frameshift. The left end of the BtI1 chain of glyphs is at the end of a contig. Colored rectangles below the 0305φ8-36 orfs indicate paralogous domains in 0305φ8-36. Open black boxes immediately under 0305φ8-36 orfs or within BtI1 orfs indicate FN3 domains. Closed black boxes indicate domains as follows: Under orf147 – T4gp27 domain, under orf163 – a C-terminal intimin domain, under orf164 – bacterial von Willebrand's factor domain, within RBTH_07677 – LysM domains. Abbreviations include: Lg. ter. – large terminase; c.f. – putative curly fiber protein gene; pr./scaf. – protease with nested scaffold gene; h.d. – putative head decoration gene; TMP – tape measure protein; hub – homologue of T4gp27; V – homologue of P2 gpV; J – homologue of P2 gpJ.

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