Relationship of VP60 capsid proteins among diverse isolates of RHDV. The predicted amino acid sequences of 45 RHDV isolates were aligned in CLUSTAL W. One thousand bootstrap replicates were subjected to protein distance and UPGMA methods and the consensus phylogenetic tree is shown. The VP60 region of a non-pathogenic rabbit calicivirus (RCV) was used as an outgroup. Two clades, one representing the original RHDV serotype and a second representing the new RHDVa subtype were identified. Bootstrap values greater than 50% are displayed above the tree branches.