Figure 2

Comparison of the binding ability of preS and preS1 to anti-preS by ELISA test. Different amounts of recombinant preS1 (white square) and preS (black square) proteins (0, 0.1 ng, 1.0 ng, 10 ng and 100 ng) were added to the polyclonal anti-preS coated plates and incubated at 37°C for 1 h, followed by incubating with HRP conjugated monoclonal anti-His (1:1000 dilution with blocking buffer). Absorbance at 450 nm was measured and compared. Samples were considered positive when the OD value was over 0.15 after subtracting the background.