Universal primers for HBV genome DNA amplification across subtypes: a case study for designing more effective viral primers

Table 2 Primers for full length genome amplification and fragment amplification

Primers	Sequence	Location*	Length	GC %	Tm(°C)	Amplicon Size (bp)
WA-L	ACTGTTCAAGCCTCCAAGCTGTGC	40	24	54.2	60.6	3181
WA-R	AGCAAAAAGTTGCATGGTGCTGGT	3221	24	45.8	60.7
FA1-L	TTTCACCTCTGCCTAATCATCTC	4	23	43.5	52.0	1014
FA1-L'	TTT ACCTCTGCCTAATCATCTC	4	22	40.9	47.5
FA1-R	TCTTGTTCCCAAGAATATGGTG	1018	22	40.9	51.0
FA2-L	GCGTCGCAGAAGATCTCAAT	593	20	50.0	51.9	1074
FA2-R	TTGAGAGAAGTCCACCACGAG	1667	21	52.4	51.7
FA3-L	CTGCTGGTGGCTCCAGTT	1451	18	61.1	50.6	1059
FA3-R	GCCTTGTAAGTTGGCGAGAA	2510	20	50.0	52.2
FA4-L	GTATTGGGGGCCAAGTCTGT	2416	20	55.0	52.8	1072
FA4-L'	GTATTGGGGGCCAAATCTGT	2416	20	50.0	52.8
FA4-R	AAAAAGTTGCATGGTGCTG	3218	19	42.1	48.5

*Here we select "CTTTTTC" of X ORF as the start point. FA1-L' and FA4-L' are degenerate primers

ISSN: 1743-422X