Figure 5
From: Dengue virus serotype infection specifies the activation of the unfolded protein response
XBP1 is spliced in Dengue infected A549 cells. A549 cells were either treated with thapsigargin (TG) or infected with DENV2 and DENV1 (10 MOI) and harvested at indicated time points. Total mRNA was extracted and analyzed with XBP1 primers (top panel) or β-actin primers (bottom panel) by semi-quantitative RT PCR. The PCR products were run on a 3% agarose gel and the spliced (sXBP1), unspliced (uXBP1) and the hybrid (hXBP1) forms are shown. Thapsigargin was used as a positive control for induction of XBP1 splicing (sXBP1) and β-actin mRNA levels as loading control.