Figure 3
From: Dengue virus serotype infection specifies the activation of the unfolded protein response
Dengue infection induces the upregulation of GADD34 mRNA. (A) A549 cells were either treated with increasing concentration of thapsigargin (TG) for 1 hour or infected with DENV2 or DENV1 viruses (10 MOI) at indicated time points. GADD34 mRNA (top panel) and β-actin mRNA (bottom panel) levels were determined by semi-quantitative RT-PCR with specific primers (see Materials and Methods). (B) Densitometric quantification of GADD34 mRNA levels from (A) were normalized to β-actin mRNA levels and plotted in histograms (TG) or graphs (DEN1 and 2). The ratio of the GADD34 to β-actin of the uninfected (Mock) sample was considered as basal level (0 hour) and negative value was also represented as basel level. (C) A549 cells were infected with either 1 moi of DENV1 (MY10245) or DENV2 (TSV01). Virus production after Day 1, Day 2 and Day 3 of infection were quantified by plaque assay and expressed by PFU/ml.