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Figure 3 | Virology Journal

Figure 3

From: In vivo transcriptional targeting into the retinal vasculature using recombinant baculovirus carrying the human flt-1 promoter

Figure 3

Effect of histone deacetylase inhibitors in the expression mediated by BacFLT-GFP. (a) Representative histograms obtained by flow cytometry 48 h after transduction of BUVEC-E6E7-1 cells with 100 MOI of BacFLT-GFP and treated with increasing concentrations (shown in the figure) of butyrate or trichostatin A (TSA). The percentage of GFP+ cells (shown inside green rectangles) is reported in the insets, and was calculated by subtracting the background from mock transduced cells. (b) Fold induction in GFP expression mediated by BacFLT-GFP with increasing concentrations of butyrate or TSA. (c) Fold induction of the expression of GFP in transfected cells with the transfer plasmid pBlueFLT-GFP (see Methods) and treated with increasing concentrations of butyrate or TSA. Results from four independent experiments ± SD. NS = non significant.

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