Figure 5

vGPCR induced PGE ₂ secretion is dependent on COX-2. A) HUVEC were infected with BABE or BABE-vGPCR at time 0 and analyzed at the indicated time points for the amount of PGE₂ in conditioned medium using an EIA kit. The vGPCR-expressing HUVEC conditioned media demonstrates a time-dependent increase in PGE₂ secretion (* = p < 0.001). Graph and SEM is representative of three independent experiments. B) At 24 hours post-infection, BABE-vGPCR infected HUVEC were treated with the non-selective COX inhibitor indomethacin (Indo), the selective COX-2 inhibitor (NS-398), or the selective COX-1 inhibitor SC-560 and PGE₂ in the conditioned media was quantified at 48 hours post-infection by EIA. PGE₂ secretion is reduced in a dose-dependent manner in Indo and NS-398 treated BABE-vGPCR infected HUVEC. The COX-1 selective inhibitor SC-560 had a minimal effect on PGE₂ secreted from vGPCR expressing HUVEC. Graph indicates fold induction in PGE₂ secretion over BABE-HUVEC and is representative of three independent experiments.