Figure 4

vGPCR induces COX-2 mRNA expression. A) A COX-2 promoter-luciferase plasmid (5 μg) was co-transfected with pcDNA3 or pcDNA3-vGPCR at the indicated concentrations. These results demonstrate a dose-dependent increase in COX-2 promoter activity in vGPCR-expressing HeLa at 48 hours (* = p < 0.001). Graph and SEM are representative of three independent experiments. B) RNA was collected from BABE and BABE-vGPCR-infected HUVEC at the indicated time points and analyzed by quantitative real-time RT-PCR for COX-2 mRNA expression. The graph demonstrates a time-dependent increase in COX-2 expression in vGPCR-expressing HUVEC and represents the mean of 3 independent infections each measured in triplicate (*= p < 0.001).