vGPCR induces COX-2 expression. HUVEC were infected at time 0 with either BABE or BABE-vGPCR. Whole cell lysates were prepared at the indicated time points and analyzed by western blot for the indicated protein. BABE-vGPCR-infected HUVEC demonstrate increased COX-2 expression beginning at 24 hour post-infection (p.i.), while neither BABE nor BABE-vGPCR-transduced HUVEC express COX-1. 50 ng of ovine COX-1 and COX-2 electrophoretic standards (S) served as positive controls. β-actin expression served as a loading control. Results are representative of three independent experiments.