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Figure 5 | Virology Journal

Figure 5

From: Progressive loss of CD3 expression after HTLV-I infection results from chromatin remodeling affecting all the CD3 genes and persists despite early viral genes silencing

Figure 5

TSA/AZA treatment of HTLV-I infected WE17/10 cells. A, Representative ethidium bromide-stained gels of CD3 γ, CD3 δ and GAPDH (endogenous control) RT-PCR products from untreated HTLV-I infected CD3γ-δlo, TSA/AZA HTLV-I infected CD3γ-δlo (treated for 72 hours with 4 μM of 5'AZA and for 18 hours with 500 nM of TSA) and uninfected untreated WE17/10 cells. B, ChIP assay using anti-Ac-H4 and anti-HDAC to study the in vivo binding to the sequence surrounding the Spγ 1 /CD3γ Inr motif in TXP/XΔ3+ uninfected and in untreated and TSA/AZA treated CD3γ-δlo HTLV-I infected WE17/10 cells.

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Virology Journal

ISSN: 1743-422X