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Table 2 LNA sequences

From: Discovery of herpesviruses in multi-infected primates using locked nucleic acids (LNA) and a bigenic PCR approach

LNA (Name)

LNA (Sequence)$

Target DPOL sequence

Tm of DNA-oligomer (°C)#

Tm of LNA-oligomer (°C)#

ΔTm (°C)

LNA-PtroLCV1

5'- +a+tg+a+cg+cg+t+ag+c+c+g --NH2

PtroLCV-1

55

82

27

LNA-GgorLCV1

5'- +at+a+acgcgt+a+gcc+g+accc --NH2

GgorLCV-1

51

77

26

LNA-EBV

5'- g+atg+act+cgaag+ctgg+ccct --NH2

EBV

63

72

9

LNA-MfasLCV1

5'- g+tggcc+a+acggcc+tc --NH2

MfasLCV-1 and Cgue LCV-1

60

69

9

LNA-PhamLCV2

5'- asg+ac+acgc+a+accgg --NH2

PhamLCV-2

59

67

8

LNA-CalHV3

5'- ac+tcgcag+ta+tacca+tccg --NH2

CalHV-3

54

69

14

LNA-PtroRHV1

5'- +a+acc+t+tg+a+a+tc+tggcg+tc -- NH2

PtroRHV-1

56

72

15

  1. $All bases in LNA conformation are preceeded by +
  2. # Tm calculated with the Exiqon Tm prediction algorithm.