Possible association of A12L with other VV proteins. The anti-A12L immunoprecipitates were absorbed in IPG strips for two dimensional gel eletrophoresis (2D-gel), which were stained with Coomassie R-250. The distinguished spots were cut out and sent for either N-terminal sequencing or MS analyses (LC-ESI-Q-TOF MS). The upper panel shows the immunoprecipitates of the cells alone (Mock) while the bottom panel is WR-infected cell lysates (WR) immunoprecipitated with A12L antibody. Arrowheads are the A12L-derived peptides distinguished from mock (upper panel) and antibody alone (data not shown). The table underneath the 2D gel stains shows the summary of the total results from both analyses.