Figure 7
From: Characterization of vaccinia virus A12L protein proteolysis and its participation in virus assembly
Identification of A12L-derived peptides. BSC-40 cells were infected with WR at an MOI of 5 PFU/cell, of which cell lysates were subjected to immunoprecipitation analyses with anti-A12L. The immunoprecipitates were resolved in 12% gel, transferred to PVDF membrane, followed by Coomassie staining. The four bands in molecular weights of 20, 15, 13, and 11 kDa were cut out and sent for N-terminal sequencing. The sequence data we obtained from N-terminal sequencing is represented in the table below. Arrows indicate the peptides, which are N-terminally blocked or not enough protein to analyze the amino acid sequences.