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Table 1 DNA amplification by PCR using conserved nature of nucleotide sequences in regions of the pre-S1 through S genes followed by gel electrophoresis, ethidium bromide staining lanes 1–5 represent positive cases for genotype C, lanes 6–10 represent positive cases for genotype D and lane 11 is PCR marker (Promega, Madison, Wis. USA).

From: Hepatitis B virus (HBV) genotypes in Egyptian pediatric cancer patients with acute and chronic active HBV infection

Primer

Sequence a(position, specificity, and polarity)

First PCR

 

P1

5'-TCA CCA TAT TCT TGG GAA CAA GA-3'

(nt 2823–2845, universal, sense)

S1-2

5'-CGA ACC ACT GAA CAA ATG GC-3'

(nt 685–704, universal, antisense)

Second PCR

 

Mix A

 

B2

5'-GGC TCM AGT TCM GGA ACA GT-3'

(nt 67–86, types A to E specific, sense)

BA1R

5'-CTC GCG GAG ATT GAC GAG ATG T-3'

(nt 113–134, type A specific, antisense)

BB1R

5'-CAG GTT GGT GAG TGA CTG GAG A-3'

(nt 324–345, type B specific, antisense)

BC1R

5'-GGT CCT AGG AAT CCT GAT GTT G-3'

(nt 165–186, type C specific, antisense)

Mix B

 

BD1

5'-GCC AAC AAG GTA GGA GCT-3'

(nt 2979–2996, type D specific, sense)

BE1

5'-CAC CAG AAA TCC AGA TTG GGA CCA-3'

(nt 2955–2978, type E specific, sense)

BF1

5'-GYT ACG GTC CAG GGT TAC CA-3'

(nt 3032–3051, type F specific, sense)

B2R

5'-GGA GGC GGA TYT GCT GGC AA-3'

(nt 3078–3097, types D to F specific, antisense)

  1. aAn "M" represents a nucleotide that could be either an A or a C; a "Y" represents a nucleotide that could be either a C or a T. nt: nucleotide.